Outsourcing Facility for Research Extension

Central Sophisticated Instrumental Laboratories

 

Central Sophisticated Instrumentation Facility (CSIF) of Dadasaheb Balpande College of Pharmacy, (DBCOP) has number of sophisticated instruments which are used for materials characterization, different physical and chemical analysis, precision chemical analysis and computational analysis. CSIF has expertise faculties and laboratory technician who assist in operation and interpretation of analytical results. Laboratory facilitate various domain of research work in areas of pharmaceutical sciences including analytical, bioanalytical and pharmacokinetics and tissue distribution studies, phyto-chemistry, natural products, Novel Drug Delivery System. The department has excellent state of the art instrumental facilities to conduct the practicals as per curriculum and research to meet the current industry demands.

The main objectives of the centre are,

• Provide characterization of different kinds of materials
• High resolution imaging and precision chemical Analysis
• Giving training to different analysis of materials through university approved short term courses and seminars.
• To train the students and to make them competent for handling sophisticated instruments and result analysis

 

Dimension of Laboratory

75 square meter

Available Instruments

High Performance Liquid Chromatography (HPLC)

 

 

 

Instrument Specification

 

Make	HPLC – Shimadzu (LCAD-20), Binary Gradient
Injector	Manual rheodyne injector, Loop 20 μL
Detector	Photodiode array detector PAD range 200-900nm
Column Specification	XBridge BEH C18 Column, 130Å, 5 µm, 4.6 mm X 250mm
Guard Column	XBridge BEH C18 VanGuard Cartridge, 130Å, 5 µm, 3.9 mm X 5 mm
Dimension	260(W) x 140 H) x 420(D) mm
Software	Lab solution
Instrument cost	14,50,000/-

Facility Provided:-

1. Development of analytical and bioanalytical method of the pharmaceuticals
2. Pharmacokinetics and tissue distribution studies of the pharmaceuticals
3. Qualitative and quantitative determination of drugs and natural products.

Outsourcing charge:-

Subjected to the number of injections for trail and cost of HPLC grade solvents

 

High Performance Liquid Chromatography (HPLC)

 

 

 

Instrument Specification

 

Make	HPLC –SPD10A Analytical Technology Ltd., Isogratic
Product Number	WCX-5-300/25
Injector	Manual rheodyne injector, Loop 20 μL
Detector	UV-Visible 200-800nm
Column Specification	WCX HPLC Column, 5um, 300A, 4.6x250mm
Dimension	260(W) x 140 H) x 420(D) mm
Software	Analchrome data system (GPC)
Instrument cost	2,99,840/-

 

Facility Provided

Development of Analytical Method/Bioanalytical method. Quantification of Pharmaceuticals and laboratory experiments. Outsourcing charge:-

Subjected to the number of injections for trail and cost of HPLC grade solvents

 

Fourier Transform Infra Red Spectroscopy (FTIR)

 

 

 

Instrument Specification

 

Make	FTIR Shimadzu (Affinity)
Sampling Kit	Diffuse Reflectance (DRS)
Detector	Pyroelectric
Wavelength Range	8300 to 350 cm-1
Spectral Resolution	0.5 cm-1
Wavelength Accuracy	0.1 cm-1 to 3000 cm-1
Signal to noise ratio	50000:1 peak to peak 4 cm-1 resolution for a one minute measurement.
Wavelength Precision	Better than 0.01 cm-1 at 3000 cm-1
Instrument cost	13,07,000/-

 

Facility Provided

• Analysis of FTIR of pharmaceuticals and excipients for identification of functional group
• Drug Excipient Compatibility Test by FTIR

Outsourcing charge:- 150/- per sample

 

Gas Chromatography (GC)

Purchased under: AICTE MODROB Grant

Topic: Modernization of Pharmaceutical Analysis Laboratory for Qualitative and Quantitative Estimation of Pharmaceutical and Formulations

Principal Investigator: Dr. Amol Warokar

 

 

 

Instrument Specification

 

Make	Thermo fisher Trace (1110)
Injector	Manual injector, Split & Split less injector
Detector	Flame Ionization Detector (FID)
Column	FAME cyanopropylphenyl-based phase 30m x 0.32mm x 0.25um. TG-624 SilMS GC Columns, 1.4µm film thickness, 0.25mm I.D., 30m length.
Dimension	57 x 61 x 64 cm (H x W X D)
Instrument Cost	7,05,000/-

Facility Provided:-

• Separation and identification of mixture of fatty acids, volatile fatty acids, essential oil with reference standard
• Determination of organic solvent residue in sample
• Outsourcing charge:- 700/- per sample

 

Particle Size Analyzer (Zetasizer)

Purchased under Biotechnology Industry Research Assistance Council (BIRAC) Grant Topic: Dry Powder for Inhalation Containing Biodegradable Nanoparticles of Niclosamide to Combat COVID-19 Infection

Principal Investigator: Dr. Nilesh Mahajan

 

 

 

Instrument Specification

 

Make	Anton Paar Make-Litesizer 500
Measurement principle	Dynamic Light Scattering (DLS)
Measuring range	0.3 nm to 10 μm (particle diameter)
Measurement angles	15°, 90°, 175°
Min. concentration	0.1 mg/mL (lysozyme)
Max. concentration	50 %w/v (sample-dependent)
Min. sample volume	12 µL
Accuracy	Better than +/-2 % on NIST traceable standards
Measuring range	Better than +/-2 % on NIST traceable standards
Instrument Cost	22,58142/-

 

Facility Provided

• Particle characterization from the nano- to the micrometer range
• Determination of zeta potential, Molecular mass and refractive index measurements

Outsourcing charge:- 500/- per sample

 

Spectroflurophotometer

Purchased under: AICTE MODROB Grant

Topic: Modernization of Pharmaceutical Analysis Laboratory for Qualitative and Quantitative Estimation of Pharmaceutical and Formulations

 

Principal Investigator: Dr. Amol Warokar

 

Instrument Specification

 

Make	Shimadzu (RF-6000)
Light source	150 W Xenon lamp
Scanning Wavelength range	200 to 900 nm and 0 order
Resolution	1.0 nm or less (Emission)
Wavelength slewing speed	60,000 nm/min.
Detector	Phototube & Photomultiplier tube
Instrument cost	9,50,000/-

 

Facility Provided

• Determination of fluorophore
• Quantification of Pharmaceutical/natural products/biological.
• Enzyme Inhibition assay

Outsourcing charge:-

150/- per Sample

Quantification by linear regression method 1500/- (Note: Solvents to be provided by the user)

 

UV-VIS spectrophotometer (Analytical Technology)

Instrument Specification:-

 

Monochromator	Czerny-Turner type with 1200 lines/nm blazed grating
Light Source	Tungsten Halogen Lamp & Deuterium Lamp
Lamp interchange Wavelength	340~410 nm (Default 370 nm)
Detector	Silicon Photodiodes
Spectral Bandwidth	1 nm (190 to 1100 nm)
Wavelength Range	190 to 1100 nm
Wavelength Setting	0.05 nm
Wavelength Accuracy	±0.3 nm (For entire range) ±0.1 nm (656.1 nm)
Wavelength Repeatability	<± 0.1 nm
Instrument cost	2,50,000/-

 

Facility Provided:- Qualitative and Quantitative analysis of analytes.

Outsourcing charge:- 100/- per sample

 

 

UV-VIS spectrophotometer (Shimadzu-1800)

Instrument Specification:-

 

Bandwidth	1 nm (190 to 1,100 nm)
Dimension	490 mm X 270 mm X 450 mm
Photometric Range	Absorbance: -4 to 4 Abs, Transmittance: 0% to 400%
Weight	15 kg
Scanning Speed	3,000 to 2 nm/min
Light Source	20-W halogen lamp and deuterium lamp Built-in light source auto position adjustment
Optics	Double Beam
Photodetector	Photodiode
Photometric Noise	Less than 0.00005 Abs (700 nm)
Instrument Cost	3,73,334/-
Sample Compartment	Internal dimensions: 110 (W) x 250 (D) x 115 (H) mm Distance between light beams: 100 mm
Stray Light	Less than 0.02% at 220 nm (NaI). Less than 0.02% at 340 nm (NaNO2). Less than 1.0% at 198 nm (KCl).

 

Facility Provided:- Qualitative and Quantitative analysis of analytes.

 

Outsourcing charge:- 100/- per sample

 

Brookfield Viscometer

 

 

 

Instrument Specification

 

Viscosity	0.2 - 15,000 Poise
Shear Rate	0.2 - 15,000 Poise
Temperature	5-75°C or 50-235°C.
Speeds	LVF: 60, 30, 12, 6 LVT: 60, 30, 12, 6, 3, 1.5, 0.6, 0.3
Accuracy:	± 1% of Full Scale Range in use
Repeatability:	± 0.2% of Full Scale Range in use
Weight:	Gross Weight 20 lb 9 kg Net Weight 17 lb 7.7 kg Carton Volume 1.65 cu ft 0.05 m3
Operating Temperature	-20°C (-4°F) to 65°C (149°F)
Instrument cost	1,50,000/-

 

Facility Provided:- Determination of viscosity of semisolid preparation

Outsourcing charge:- 100/- per sample

 

Design of Experiment (DOE) Software for optimization of formulation

DOE Legal Software version 14 Features

• Factorial and fractional-factorial designs, including minimum-run designs, optimal multi- level
• Response surface designs including central composite, Box-Behnken, optimal
• Mixture designs including screening, simplex and optimal
• Custom optimal combined designs for mixture-process, double-mixture, mixture-amount
• Split-plot designs for factorial, response surface and combined mixture-process

Software cost:- 45,000

Facility Provided:- Optimization of formulation by QbD.

Outsourcing charge:- 1000/- per project

 

Compact Cooling Centrifuge Remi

 

 

 

 

Instrument Specification:-

 

Make	Remi
CM-8 Plus	CM-12 Plus
Max. Speed (rpm)	6000
Max. RCF (g)	5070
Max. Tube Size (ml)	100
Max. Capacity (ml)	400
Min. Temperature (°C)	-8
#Noise	≤ 60 dB
Votlage Stabilizers	VS – 02
Instrument Cost	99,000/-

 

Facility Provided:- Separation of plasmas , serum ,sedimentation of sample.

Outsourcing charge:- 100/- per sample

 

 

1. Analytical Electronic Balance (0.1 mg Sensitivity)

Instrument Specification

 

• AC Operating Voltage – 230V, 50 Hz, 10%

• DC Operating Voltage -9 V, 1 Amp
• Operating Temperature – 0 to 40 Celsius
• Auto Tracking Zero
• Auto Power Saving Feature
• LCD Display
• 100% overload protection architecture
• A to D converter with 40 pin programmable IC
• 32-bit expandable architecture with Advanced PLU logic systems
• Digital overload indication with 100% tare functionality
• Digital auto Calibration
• Printer output is Provided

 

Facility Provided:- Accurate weighing of analyte

Outsourcing charge:- 50/- per sample

 

 

Franz Diffusion Cell for Drug Permeability Studies

Instrument Specification

 

Make	Orchid Scientific
Magnetic Stirrer	8 Stage
Material	Stainless Steel 304
RPM Indicator	Digital
RPM Range	60-990
Water Circulation System	Stainless Steel 304
Temp. Range & Accuracy	5°C Above ambient to 80°C (±0.2°C)
Clamps For Holding Diffusion Cell	08
Diffusion Cells	08
Magnetic Beads	08
Boss Heads, Clamps	08
Millipore type Clamps	08

 

Facility Provided

• Drug permeation studies of topical preparation
• Transdermal permeability and transmucosal permeability studies

Outsourcing charge:- 300/- per sample